The government of Aung San Suu Kyi has already said it would refuse to cooperate with a UN mission following a resolution adopted by the Human Rights Council in March.
Kyaw Zeya, permanent secretary at the Ministry of Foreign Affairs, said: "If they are going to send someone with regards to the fact-finding mission, then there's no reason for us to let them come."
Zeya added that visas to enter Burma would not be issued to any staff working on the mission.
The Burmese government previously brushed away evidence of human rights violations as fake news and "propaganda".
It also deemed "exaggerated" a UN report published in February which found babies and children were reportedly slaughtered with knives amid "area clearance operations".
The report concluded counter military operations by security forces were subjecting the Rohingya population to brutal beatings, disappearances, mass gang rape and killings.
Suu Kyi, who came to power last year as a part of a transition from military rule, has been criticized for failing to stand up to the more than one million stateless Rohingya Muslims.
In March, the EU called for a mission to look into the abuses in the north of the country.
Indira Jaising, an advocate from the Supreme Court of India, was appointed to lead the mission in May.